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. 2011 Dec 15;22(24):4892–4907. doi: 10.1091/mbc.E11-04-0371

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© 2011 Kuravi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Bck2 localization significantly changes during heat shock. (A) Representative wide-field fluorescence images of Bck2-GFP cells (FLY3503) 90 min after 15 mM caffeine treatment at 22°C and 30 min after cells were transferred to 39°C (heat shock). Caffeine treatment does not induce changes in Bck2 localization, but heat shock causes Bck2 to relocate from the nucleoplasm to bright cytoplasmic puncta. (B) Time-lapse analysis of heat-shocked Bck2-GFP cells. Bck2-GFP cells were heat shocked at T = 0 and images were captured at 30-s intervals. Arrowheads point to representative nuclear foci that remain visible throughout the experiment. These data are also presented in Supplemental Movie S1. (C) Moderately overexpressed Bck2-GFP (from pGPD-BCK2-GFP) does not localize to mitochondria or other cytoplasmic puncta during heat shock (images were captured 15 min after heat stress induction). All images were captured via wide-field fluorescence microscopy. All images in A and C represent single optical sections, and the images in B are merged from 3 × 0.2 μm optical sections.