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. 2011 Dec 12;61(1):124–136. doi: 10.2337/db11-0998

FIG. 2.

FIG. 2.

BAMBI manipulation affects priming of SGBS PAs. The effect of BAMBI knockdown/overexpression on priming of SGBS PAs was assessed using RT-PCR, Western blot, and microscopy of cells 72 h posttransfection with SCR or BAMBI siRNA or 72 h with FGF-1 (A–D) or transfection with GFP or BAMBI cDNA (E and F). A: BAMBI mRNA expression was assessed by RT-PCR. Results are expressed as fold compared with SCR (control) cells (mean ± SEM; n = 7; ANOVA, ***P < 0.001). B: BAMBI protein expression was assessed by Western blot (β-tubulin was used as a loading control). A representative blot (top) and quantitation of data from three to six independent experiments (bottom) are shown. Results are expressed as fold compared with SCR (control) cells (mean ± SEM; ANOVA, ***P < 0.001). C: Photomicrographs of SGBS PA cells showing cell morphology (images are representative of seven independent experiments). D: PPARγ mRNA expression was assessed relative to SCR (control) cells, by RT-PCR (ANOVA, *P < 0.05; **P < 0.01). E and F: BAMBI and PPARγ expression assessed by RT-PCR. Results are expressed as fold compared with SCR (control) cells (mean ± SEM; n = 4–7; ANOVA, *P < 0.05). (A high-quality color representation of this figure is available in the online issue.)