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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Cancer Cell. 2011 Nov 15;20(5):620–634. doi: 10.1016/j.ccr.2011.10.001

Figure 2. Cyclin D-CDK4/6 Activate FOXM1 Transcriptional Function.

Figure 2

(A) Effects of individual and combined expression of FOXM1 and cyclin D-CDK4/6 complexes on reporter gene activation (+/−SD). Hela cells were transfected with the CCNG2, TSC22D1, IGFBP-1, 6DB, CENPF and PLK1 promoter-luciferase constructs, FOXM1 (M1), empty vector (-), cyclin D1-CDK4 (14) or cyclin D3-CDK6 (36).

(B) Reporter activity (+/−SD) in Hela cells after transfection of FOXM1 (M1) and cyclin D1-CDK4 (14), or cyclin D3-CDK6 (36), versus kinase-dead (KM) versions.

(C) U2OS cells stably expressing either control (sh-con) or RB1 targeting shRNA (sh-RB1) were transfected with the CCNG2 promoter-reporter, empty vector (EV, blue) or FOXM1 (pink), and treated with DMSO, 0.1 μM or 1 μM PD0332991 for 16 h. Values are mean +/−SD.

(D) RB1 protein expression in control (sh-con) and RB1 knockdown (sh-RB1) cells.