Table 1.
Percent Se species in A549 cells treated with selenite, as estimated by a linear combination of model compound spectra.a
| Treatment (μM) | Time (h) | Cell Viability (%) | Percentage (%) of component fitted
|
Ntot | residual (× 10−3) | |||
|---|---|---|---|---|---|---|---|---|
| SeCys | CysSeSeCys | GSSeSG | α-Se | |||||
| 1 | 24 | 95 ± 3 | - | 98(3)b | - | - | 0.98 | 1.69b |
| 5 | 1 | n. d.c | - | 68(5)b | 33(5)b | - | 1.01 | 2.22b |
| 5 | 4 | 98 ± 5 | 37(1) | - | 26(4) | 37(3) | 1.00 | 0.42 |
| 5 | 24 | 85 ± 6 | 34(2) | - | 23(5) | 44(3) | 1.01 | 0.55 |
| 5 | 48 | 53 ± 8 | - | 19(4) | 26(6) | 55(3) | 1.00 | 0.70 |
| 5 | 72 | 51 ± 1 | 12(3) | 13(6) | 27(4) | 49(3) | 1.01 | 0.31 |
a
Values in parentheses are the estimated standard deviation derived from the diagonal elements of the covariance matrix and are a measure of precision. Ntot is the sum of the fractions.
b
High residuals (>1 × 10−3) indicate poor fits that do not give an accurate indication of Se speciation.
c
Cell viability was not determined at this time point.