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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Exp Hematol. 2011 Oct 20;40(1):84–91. doi: 10.1016/j.exphem.2011.10.001

Figure 1. Transient silencing of PTEN expression in human CD34+ cells by siRNA.

Figure 1

(A) Western blot analysis of human CD34+ cell protein extracts 1 to 10 days following PTEN or control siRNA transfection. Untreated CD34+ cells served as a positive control. GAPDH was used as a control for equal loading of samples. The vertical lines indicate grouping of images from different gels. (B) Real-time PCR analysis of untreated human CD34+ cells, and CD34+ cells transfected with PTEN or control siRNA 48hours after transfection. Data are shown as mean percentages (± SD, n=5, t test) relative to untreated cells. mRNA levels were normalized to GAPDH expression. *p<0.01; **p<0.0001