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. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: J Immunol. 2011 Nov 11;187(12):6499–6507. doi: 10.4049/jimmunol.1101398

Figure 4. Phenotype of TGF-β-induced LAP+Foxp3− T cells.

Figure 4

LAP+Foxp3− and LAP−Foxp3+ OT-II T cells were generated as described in Fig. 3. (a) Representative expression of CD25, GITR, CD103, Granzyme B and CTLA-4 (black line), with isotype controls (gray shade), at day 4 on gated LAP+Foxp3− and LAP−Foxp3+ CD4+ T cells. (b) Cytokine secretion profiles of TGF-β-induced sorted LAP+Foxp3−, LAP−Foxp3+, and LAP−Foxp3− T cells. Positive control populations (Effector cells) were from cultures of OT-II splenocytes stimulated with OVA peptide in non-skewing conditions for 4 days. T cells were restimulated with PMA (50 ng/ml) and Ionomycin (1 μg/ml) for 24h. IL-2, IL-4 and IFNγ levels were measured using mouse cytokine multiplex kits. Similar data were obtained in 3 independent experiments. *P < 0.05.