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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2011 Oct 6;10(12):2257–2266. doi: 10.1158/1535-7163.MCT-11-0149

Figure 2. H929-60 cells have reduced α4 integrin protein levels and reduced adhesion to FN and VCAM-1.

Figure 2

A) Surface expression of α4 and β1 integrin on H929 and H929-60 cells was determined by FACS analysis (* denotes p<.05 n=9, Student's t-test). B) Whole cell lystates of H929 and H929-60 cells were probed for α4 integrin, β1 integrin and beta actin by Western blot analysis. α4 integrin mRNA levels were determined using real time rt-PCR. α4 integrin mRNA expression levels were normalized by dividing by GAPDH levels (p>.05 n=3). C-D) H929 and H929-60 cells were incubated with α4 blocking antibody or IgG control antibody for 30 minutes and subsequently adhered to (C) FN (40 μg/ml) or (D) VCAM-1 (10 μg/ml) coated plates for 2 hours. A representative of each experiment is shown (* denotes p<.05 or # denotes p>.05 Student's t-test).