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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2011 Oct 6;10(12):2257–2266. doi: 10.1158/1535-7163.MCT-11-0149

Figure 3. Reducing the expression of α4 and β1 integrins caused partial resistance to HYD1 induced cell death in H929 and 8226 cells.

Figure 3

A) and C): In H929 cells α4 and β1 integrin expression was reduced via transient infection. A: At 72 hours post infection of shRNA, α4 and β1 integrin expression was determined by FACS analysis. 8226 cells were stably infected with α4 integrin shRNA, β1 integrin shRNA or control vector shRNA. FACS analysis was used to determine expression of α4 and β1 integrin. Shown is a representative histogram from one experiment. B) and D): H929 and 8226 cells were treated with 50 μg/ml and 100ug/ml of HYD1 respectively for 6 hours. After 6 hours, cell death was analyzed by Topro-3 staining and FACS analysis. (* denotes p<.05 n=9, Student's t-test).