Skip to main content
. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2011 Oct 6;10(12):2257–2266. doi: 10.1158/1535-7163.MCT-11-0149

Figure 5. H929-60 cells display reduced binding to HS-5 and MSC and are not resistant to bortezomib or melphalan in the stroma co culture model.

Figure 5

A-B) H929 and H929-60 pre-incubated with CMFDA dye were adhered to 10,000 HS-5 or MSC cells for 2 hours. After 2 hours unadherent cells were removed and the fluorescence intensity was measured on a fluorescent plate reader. C-D): H929 and H929-60 cells were adhered to HS-5 GFP cells for 24 hours. After 24 hours samples were treated with 15 μM melphalan or 16 nM bortezomib for 24 hours. Cell death was analyzed using a FACScalibur with the GFP expressing HS-5 cells being excluded from the analysis. In A-C), a representative of three independent experiments was shown. (* =p<.05, n=9 or# =p>.05, n=9 Students t-test).