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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Shock. 2012 Jan;37(1):70–76. doi: 10.1097/SHK.0b013e31823180a4

Fig. 2. Insulin signaling in liver following trauma and hemorrhage for 90 min with macrophage depletion by GdCl3.

Fig. 2

Rats were injected with GdCl3 (10 mg/kg) via the tail vein, at 48 hours post-injection, surgical trauma and hemorrhage for 90 min was performed and liver tissues were harvested. (A). Liver protein extracts were subjected to Western Blot analysis with anti-PS473-Akt and total-Akt antibodies. (B) The level of PS473-Akt was presented as the mean +/− SEM in the graph (n=3 for each group); (C) Liver protein extracts were subjected to Western Blot analysis with anti-PY972-IR, PY612-IRS1, total-IRS1 and total-IR antibodies. (TH90’: trauma and hemorrhage for 90 min; T90’: trauma alone for 90 min). The levels of PY972-IR (D) and PY612-IRS1 (E) was presented as the mean +/− SEM in the graphs (n=3–5 for each group).