Rats were injected with GdCl3 (10 mg/kg) via the tail vein, at 48 hours post-injection, surgical trauma and hemorrhage for 90 min was performed and liver tissues were harvested. (A). Liver protein extracts were subjected to Western Blot analysis with anti-PT181/Y183-JNK1/2, PS181-IKKβ/PS180-IKKα, and total-ERK antibodies. (B, C, D). The levels of P-JNK1 (B), P-IKKα (C) and P-IKKβ (D) in liver was presented as the mean +/− SEM in the graph (n=4–9 for each group; TH90’: trauma and hemorrhage for 90 min; T90’: trauma alone for 90 min).