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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Shock. 2012 Jan;37(1):70–76. doi: 10.1097/SHK.0b013e31823180a4

Fig. 7. Partial recovery of hepatic insulin signaling following trauma and hemorrhage for 210 min after splenectomy.

Fig. 7

Splenectomy was performed on rats at 10 days before or immediately prior to the surgical trauma and hemorrhage procedure. After a hemorrhage period of 90 min, resuscitation over 60 min and recovery in the cage for 60 min, liver tissues were harvested. (A). Liver protein extracts were subjected to Western Blot analysis with anti-PS473-Akt, PY972-IR, PY612-IRS1, and total-Akt antibodies. The levels of PY972-IR (B), PY612-IRS1 (C), and PS473-Akt (D) are presented as the mean +/− SEM in the graph (n=2–4 for each group; TH210’: trauma and hemorrhage for 90 min + resuscitation for 60 min + recovery for 60 min; T210’: trauma alone for 210 min; #: P<0.05 vs T210’+ins in control or splenectomized groups respectively; * : P<0.05 vs TH210’ +ins in non-splenectomized control group; &: P=0.064 vs TH210’ +ins in non-splenectomized control group; $: P=0.055 vs TH210’ +ins in non-splenectomized control group).