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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Traffic. 2011 Oct 17;13(1):25–29. doi: 10.1111/j.1600-0854.2011.01287.x

Figure 2. Application of QD quenching to study protein trafficking.

Figure 2

A) Schematic representation of the experimental strategy. B) Examples of BCG quenching of QD fluorescence at different emission wavelengths (scale bar, 2 µm). (insets) Area integrated fluorescence intensities for QD (black, red, grey and blue) and background areas (dashed line). C) β2AR recycling after stimulation and agonist wash-out measured by QD quenching and by (inset) TIRF imaging of Alexa Fluor 488-labelled receptors. D) CFTR endocytosis (left) and recycling (right) rates quantified by QD quenching. E) Real time visualization of CFTR and β2AR recycling assessed by QD quenching (scale bar, 1 µm). (insets) QD blinking indicates individual QDs were imaged. F) BCG cell permeability. MDCK-CFTR-3HA cells labelled with 605 nm QDs that were internalized were imaged at indicated times following addition of 100 µM BCG (scale bar, 2 µm).