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. Author manuscript; available in PMC: 2013 Jan 10.
Published in final edited form as: Regul Pept. 2011 Oct 1;173(1-3):36–46. doi: 10.1016/j.regpep.2011.09.003

Figure 4.

Figure 4

Hepatic expression of genes involved in FA processing in Iqgap2-/- and wild-type mice. Quantitative RT-PCR was completed using oligonucleotide primers specific for: fatty acid transport proteins (fatp1-5), fatty acid binding proteins (fabp 1-5), caveolin-1 (cave1), FAT/CD36, acyl-Coenzyme A oxidase 1 (Acox1), carnitine palmitoyltransferase 1a (Cpt1a) and sterol regulatory element-binding protein SREBP-1c and SREBP-2 genes (see Table 1). The relative transcript expression was normalized to actin expression, calculated using the comparative threshold cycle number (Δ-Ct method) and expressed as relative transcript abundance in ng mRNA per 1 ng of β-actin mRNA. Mean expression levels from triplicate wells and standard deviation were log10-transformed and plotted on a logarithmic scale.