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. Author manuscript; available in PMC: 2013 Jan 10.
Published in final edited form as: Regul Pept. 2011 Oct 1;173(1-3):36–46. doi: 10.1016/j.regpep.2011.09.003

Figure 5.

Figure 5

Expression levels of lipogenesis-related genes in IQGAP2-deficient livers. (A) Hepatic FAS, PPARα and PEPCK RNA transcript levels measured by qRT-PCR. RNA transcript levels of fatty acid synthase (FAS), peroxisome proliferator-activated receptor α (PPARα) and phosphoenolpyruvate carboxykinase (PEPCK) were measured in response to a single intraperitoneal insulin injection (5U/kg) by qRT-PCR. The relative transcript expression was calculated as described for Figure 4. (B) Immunoblot confirming protein expression of FAS, FABP1 and caveolin-1 in wild-type, Iqgap2-/- and Iqgap1-/-/Iqgap2-/- livers. The blots are representative of three independent experiments analyzing three different groups of mice. (C)Densitometric quantification of hepatic FAS protein expression normalized to GAPDH levels.