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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Microbiol. 2011 Nov 10;82(6):1433–1443. doi: 10.1111/j.1365-2958.2011.07900.x

Figure 2. Quantification of EGJ or Golgi copy number through the trypanosome cell cycle.

Figure 2

Log phase BSF TbSec23.2HA/TbGT15Ty cells were stained for the EGJ, and log phase PCF cells were stained for the Golgi, as described in Figs. 1 & 5, respectively. BSF EGJ (Panel A) or PCF Golgi (Panel B) copy numbers were determined with >500 BSF or >300 PCF cells scored in in each of three independent experiments. For BSF cells only anti-HA+/anti-Ty+ structures aligned with the FAZ filament were scored. For PCF cells only anti-TbGRASP-positive structures aligned with the FAZ filament were scored. Data are presented as percent of each kinetoplast/nuclear configuration (1K1N, 2K1N, 2K2N; mean ± s.e.m.) comprised by each EGJ or Golgi copy number. Numbers in parentheses indicate the percent of total cells in each stage of the cell cycle (mean ± s.d.).