Figure 7. TLR4 deficiency does not impair development of Ti-induced Th2-type innate immune response.
TLR4 deficient C3H/HeJ and control C3H/OuJ mice were inoculated ip with Ti or vehicle. Forty-eight hours later peritoneal exudate cells were collected and cell suspensions pooled from each treatment group. Pooled samples were stained for CD11b and Ly6g to detect neutrophils (A), c-kit and Siglec F to detect eosinophils (B), and F480 and CD206 to detect alternatively activated (M2) macrophages (C). This experiment was repeated two times with similar results.