a) Molecular structures of the thiazole antibiotic, thiostrepton, and DSPE-PEG2000-MeO, the PEGylated amphiphilic lipid used encapsulate thiostrepton into micellar nanoparticles. b) Representation of micelle-thiostrepton nanoparticles, depicting the solubilization of hydrophobic thiostrepton molecules in the inner hydrophobic compartment of the micelle nanoparticle. The nanoparticle is formed by the self assembly of DSPE-PEG2000-MeO and thiostrepton in aqueous solutions. c) Formulation studies to identify the lowest lipid-to-drug ratio required to completely encapsulate 1mM of thiostrepton. 3:1 lipid/drug ratio m/m was identified as the most acceptable ratio for further study. All measurements represent the average of 3 separate experiments, and error bars represent standard deviations.