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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2011 Sep 8;10(12):2287–2297. doi: 10.1158/1535-7163.MCT-11-0536

Figure 6.

Figure 6

a) Western blotting of a panel of individual HepG2 tumor homogenates (6 of non-treated and 6 of micelle-thiostrepton-treated) against FOXM1, cleaved caspase-3 and β-actin. FoxM1 expression is evidently suppressed and cleaved caspase-3-associated cell apoptosis is attenuated in tumors treated with micelle-thiostrepton. b) Immunhistochemical analysis of 4 individual HepG2-luc tumors (2 from non-treated and 2 from treated groups) for FOXM1 and cleaved caspase-3 expression (counterstained with hematoxylin). FOXM1 expression is found to be at higher densities in non-treated tumors, compared to thiostrepton-treated tumors. Cleaved-caspase-3 is evidently enhanced in tumors treated with micelle-thiostrepton, identified by the presence of larger numbers of dark, DAB-stained clusters. Control slides were incubated with IgG primary antibodies. Blue bars represent 100μm (20×).