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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Cell Biochem. 2012 Jan;113(1):220–228. doi: 10.1002/jcb.23347

Fig. 3.

Fig. 3

Rapamycin inhibits Epo-dependent and -independent osteoclastogenesis in mouse marrow mononuclear cells. Mouse marrow mononuclear cells (mMMCs) were treated with Epo and/or rapamycin while cultured in media with/without RANKL and M-CSF. After 1 week, TRAP staining was performed. (A) Representative micrographs of TRAP-positive osteoclast. Original magnification at 100x. (B) The numbers of TRAP-positive osteoclast were scored. The results represent average values from triplicate assays and the experiments were performed twice. (C) Representative micrographs of bone resorption. Original magnification at 100x. (D) Resorption area from five randomly selected fields was quantified using Image J software.