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. Author manuscript; available in PMC: 2012 Dec 9.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Nov 6;416(1-2):45–50. doi: 10.1016/j.bbrc.2011.10.133

Figure 1. STIM1 and Orai1 are expressed in HL-1 cardiomyocytes.

Figure 1

Panel A, RT-PCR was conducted using RNA isolated from HL-1 cardiomyocytes with the use of primers designed for mouse STIM1 and Orai1 (expected sizes, 140 and 150 bp respectively). There was no significant amplification in the no-template control (NTC) or the no-reverse transcriptase (NRT; Taq polymerase only) samples (HW Lad= 100 bp ladder; LW Lad= 50 bp ladder). Panel B, Western blot using STIM1, Orai1, and β-tubulin antibodies and total protein isolated from HL-1 cardiomyocytes as well as a positive control (skeletal muscle).