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. Author manuscript; available in PMC: 2012 Dec 29.
Published in final edited form as: Neuroscience. 2011 Oct 1;199:535–547. doi: 10.1016/j.neuroscience.2011.09.052

Figure 6. Photomicrographs showing immunofluorescent co-localization of caspase-3 and NeuN, calbindin, and calretinin.

Figure 6

(A,D,G,J) Photomicrographs showing representative cells which express cleaved caspase-3 (red). (B) NeuN, (E) calbindin, and (H) calretinin positive cells (green). DAPI nuclear label is shown in blue (K). Merged photographs which reveal co-localization of caspase-3 with NeuN, calbindin, calretinin, and DAPI (C,F,I,L). Note the relatively weak NeuN labeling of the caspase-3 positive cell (B) indicating a decreased NeuN phenotype in this apoptotic neuron. Images are captured using a 40× objective. White arrow indicates co-localized cells; yellow arrow indicates a caspase-3 positive cell that is not co-localized; red arrow indicates a fragmented nucleus with condensed chromatin.