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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Cell Signal. 2011 Sep 22;24(2):435–442. doi: 10.1016/j.cellsig.2011.09.016

Figure 2.

Figure 2

Ang II increases SGK1 mRNA levels via mobilization of intracellular calcium and tyrosine kinase activation. A, Cells were serum starved overnight and pre-treated with BAPTA-AM (50 μM, 10 min) or verapamil (10 μM, 5 min) prior to treatment with Ang II for 0 or 2 h. SGK1 mRNA was quantified via qRT-PCR. n=3. *, p<0.05 vs. 0 h control. B, Cells were serum starved overnight and pre-treated with PD98059 (50 μM, 1 h), Go6983 (10 μM, 1 h), or genistein (100 μM, 1 h) prior to treatment with Ang II for 0 or 2 h. SGK1 mRNA was quantified using qRT-PCR. n=3. *, p<0.05 vs. 0 h control.