Table 9.
Approach 5 – calculating spatial distribution of gold particles in 10 epithelial cells sampled using the rotator
| Observed gold particles, Ngo | Sum of distances, Σdv | ||||||
|---|---|---|---|---|---|---|---|
| Class | Mid-class point (μm) | RER | Golgi complex | Granules | RER | Golgi complex | Granules |
| 1 | 0.3 | 11 | 78 | 53 | 3.3 | 23.4 | 15.9 |
| 2 | 0.9 | 29 | 22 | 31 | 26.1 | 19.8 | 27.9 |
| 3 | 1.5 | 19 | 31 | 2 | 28.5 | 46.5 | 3.0 |
| 4 | 2.1 | 1 | 34 | 17 | 2.1 | 71.4 | 35.7 |
| 5 | 2.7 | 0 | 1 | 0 | 0 | 2.7 | 0 |
| 6 | 3.3 | 21 | 29 | 0 | 69.3 | 95.7 | 0 |
| 7 | 3.9 | 25 | 8 | 3 | 97.5 | 31.2 | 11.7 |
| Column total | 106 | 203 | 106 | 226.8 | 290.7 | 94.2 | |
| Ng = Σdv × π/0.07 | 10178.8 | 13046.6 | 4227.7 | ||||
| Ng/cell = Ng/10 | ∼ 1018 | ∼ 1305 | ∼ 423 | ||||
Distances in μm from a vertical axis (orthogonal to epithelial basal lamina and passing through the nucleolus) were classified using equidistant (0.6-μm) intervals. Section thickness was 0.07 μm.