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. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: Biochemistry. 2011 Nov 23;50(50):10910–10918. doi: 10.1021/bi201439m

Figure 4.

Figure 4

Resonance Raman Spectra of the ferrous derivative of the T342A mutant (A) and wild type (B) hTDO, in the substrate-free (SF), L-Trp-bound form or D-Trp bound form. The spectra of the SF and L-Trp-bound form of the wild type enzyme are taken from literature.12 The Trp concentration used for generating the substrate-bound enzyme was 20 mM. The peak at 757 cm−1 indicated by an asterisk (*) is derived from Trp substrate.