. Author manuscript; available in PMC: 2012 Dec 20.

Published in final edited form as: Biochemistry. 2011 Nov 23;50(50):10910–10918. doi: 10.1021/bi201439m

Table 2.

Michaelis-Menten parameters of the wild type and T254 mutant of hTDO. All parameters are taken from the data presented in Figure 5.

hTDO	Substrate	Km (mM)	kcat (s⁻¹)	kcat/Km
Wild type	L-Trp	0.12±0.02	2.24 ± 0.08	18.7
	D-Trp	0.20±0.06	0.26 ± 0.02	1.3

T342A	L-Trp	1.19±0.09	0.101±0.002	0.08
	D-Trp	1.59±0.13	0.082±0.002	0.05