Table 1.

First dimension operational parameters and peak capacities.

	Analysis Time [min]
	15		30		60
Split Mode	split-less	split	split-less	split	split-less	split
1tg [min] a	6	12.4	19	25	43	52.1
1tre-eq [min] b	9	2.6	11	5	17	7.9
1λ c	0.40	0.82	0.63	0.83	0.72	0.87
1λ Ratio d	2.05		1.31		1.20
1L [cm] e	5	20	10	30	25	40
1F [μL/min] f	100	570	100	380	100	290
Vinjection [μL] g	1.5	8.57	1.5	5.7	1.5	4.35
Injection Delay [min]	4.9	0.86	4.9	1.29	4.9	1.69
1φi h	0	0	0	0	0	0
1φf i	0.81	0.49	0.62	0.50	0.63	0.47
1wave.[s]	9.7	4.99	12.9	8.28	16.1	13.4
1wave.Ratio j	1.94		1.55		1.20
1nc, pred k	46	228	94	275	149	317
1nc, pred Ratio l	5.0		2.9		2.1
1nc, measured m	37	149	88	181	157	232
1nc, measured Ratio n	4.0		2.1		1.5

Column is Zorbax SB-C3 2.1 mm i.d. 3.5 μm particles. Temperature is 40 °C.

^aGradient time;

^bRe-equilibration time;

^cFraction of the analysis time devoted to the separation;

^dRatio of ¹λ value for split to split-less mode;

^eColumn length;

^fFlow rate;

^gInjection volume;

^hInitial eluent strength;

ⁱFinal eluent strength;

^jRatio of average peak width for split-less to split mode;

^kPredicted peak capacity obtained with the optimization procedure;

^lRatio of predicted peak capacity for split to split-less mode;

^mMeasured peak capacity calculated with eq. (7) using average peak widths;

ⁿRatio of measured peak capacity ratio for split to split-less mode.