Skip to main content
. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: Anal Chem. 2011 Nov 23;83(24):9531–9539. doi: 10.1021/ac202317m

Table 2.

Second dimension operational parameters and peak capacities.

Cycle Time [s]
12 21

2tg [s] a 9 18
2tre-eq [s] b 3 3
2L [cm] c 3.3 3.3
2F [mL/min] d 3.0 3.0
Vloop [μL] e 20 34
2φi f 0 0
2φf g 1.0 1.0
2nc, measured h 24 32

Column is 3.3 cm long by 2.1 mm i.d. packed with ZirChrom-CARB 3 μm particles. Temperature is 110 °C.

a

Gradient time;

b

Re-equilibration time;

c

Column length;

d

Flow rate;

e

Sample loop volume;

f

Initial eluent strength;

g

Final eluent strength;

h

Second dimension peak capacity calculated by eq. (8) using measured average peak width.