Fig. 2.
Ang II stimulation reduced the expression of nephrin and phospho-nephrin in podocyte. (A): Immunofluorescence detection of glomerular nephrin expression in different groups. Original magnification ×400; (a): normal control group on day 14; (b): Ang II-infused group on day 14. (c): Telmisartan treatment group on day 14; (d): normal control group on day 28; (e): Ang II-infused group on day 28. (f): Telmisartan treatment group on day 28; (B): immunofluorescence detection of nephrin expression in cultured podocytes stimulated by Ang II (10−6 mol/L) at various time points or pretreated with losartan (10−5 mol/L). Original magnification ×400; (C): Western-blotting detection of phospho-nephrin and nephrin expression in cultured podocytes stimulated by Ang II (10−6 mol/L) at various time points. (D): Western-blotting detection of phospho-nephrin and nephrin expression in cultured podocytes pretreated with losartan (10−5 mol/L) before stimulated by Ang II (10−6 mol/L) for 6 h. In C and D, n=6. *p<0.05 compared with control group; #p<0.05 compared with Ang II treated group.