Figure 2. Eotaxin elicits production of biologically active PGD₂.

Both human and mouse eosinophils were pre-treated for 30 min with HQL-79 before eotaxin stimulation. In A and B, for analysis of PGD₂ and cysLTs synthesis, human eosinophils were stimulated for 1 h with eotaxin (6 nM) and then challenged with A23187 (0.1 μM). In C, for analysis of lipid body biogenesis, human eosinophils were stimulated for 1 h with eotaxin (6 nM). In D, confocal images of EicosaCell preparations display intracellular immuno-detection of newly formed PGD₂ (green) in human eosinophils stimulated with rh eotaxin (6 nM). Overlay images of immuno-fluorescence and light microscopy of identical fields are shown in the larger images. In E, for analysis of PGD₂ synthesis, mouse eosinophils were stimulated for 1 h with rm eotaxin (6 nM). Panel F shows confocal images of PGD₂ immuno-detection of H-PDS (green) in mouse eosinophils stimulated with rm eotaxin (6 nM). Blue fluorescence shows eosinophil nuclei stained with DAPI. Bar, 5 μm. Results are expressed as means ± SEM for at least three independent experiments with eosinophils from distinct donors. ⁺ P ≤ 0.05 compared with control. ^* P ≤ 0.05 compared with eotaxin-stimulated eosinophils.