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. Author manuscript; available in PMC: 2012 Dec 6.
Published in final edited form as: Curr Biol. 2011 Nov 23;21(23):1979–1987. doi: 10.1016/j.cub.2011.11.002

Figure 4.

Figure 4

Role of PI4K in the activation of TRPC6 by WNK1. (A) Effect of wortmannin (Wmn) on whole-cell TRPC6 currents activated by WNK11–491. Cells were pretreated with 10 µM wortmannin or vehicle (DMSO) for 10 min before recording. *, p < 0.01, vehicle vs wortmannin. (B) Whole-cell TRPC6 currents in response to WNK11–491 co-applied with monoclonal anti-PI4P antibody or control mouse IgM. *, p < 0.01, control IgM vs PI(4)PAb. (C) Whole-cell TRPC6 current in response to intracellular delivery of 100 nM PI4KIIIβ preincubated with or without 10 µM wortmannin for 10 min. *, p < 0.01, vehicle vs wortmannin. Note that both type IIIα and IIIβ PI4K can generate PI4P for synthesis of PIP2 in the membrane. Due to difficulties in purification of PI4KIIIα, we used only PI4KIIIβ for experiments here. In all panels, data point is mean ± SEM of inward current density, n= 5–9 for each condition. (see also Figure S4).

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