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. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: Biochemistry. 2011 Nov 21;50(50):10974–10985. doi: 10.1021/bi201508t

Figure 5.

Figure 5

Spectral analysis of CcmF His261Cys (H261C) and wild-type CcmF (WT) proteins. Coomassie stain (A) and heme stain (B) of CcmF(WT) and CcmF(H261C) proteins. UV/Vis absorption spectra of CcmF(WT) (C), CcmF(H261C) (D), and CcmF(H261C) in the presence of 30 mM imidazole (E), oxidized (solid lines) and dithionite-reduced (dotted lines). Absorption maxima are indicated with arrows. Absorbance values between 500 nm and 700 nm have been multiplied by a factor of 3. 30 μg of purified protein was analyzed by Coomassie and heme stain (A, B). For UV/Vis absorption spectra approximately 20 μM purified protein was analyzed for wild-type, and approximately 40 μM purified protein was analyzed for His261Cys in the presence and absence of 30 mM imidazole.