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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Microb Pathog. 2011 Oct 18;52(1):41–46. doi: 10.1016/j.micpath.2011.09.009

Fig. 6. MicA expression in Y. pestis and functional consequences of its overexpression.

Fig. 6

(A) Total RNA was isolated from the indicated Y. pestis strains and analyzed by Northen blot analysis using a MicA specific probe. (B) Either the empty vector or MicA-encoding plasmids were transformed into wild-type Y. pestis KIM5 and the levels of MicA transcript and OmpA protein levels in the resulting transformant strains were measured by primer extension and western analysis, respectively (α-HNS: loading control). (C) RAW 264.7 cells were infected with Y. pestis transformed with either empty vector or the MicA-encoding plasmids and analyzed as described in Fig. 1. One of three independent experiments is shown.