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The general workflow was as follows: a. Rat PaSC were grown in DMEM media either with or without fetal bovine serum. b. Cells were lysed and crudely fractionated into cytoplasmic and membrane-rich protein fractions. c. The isolated protein fractions were separated by SDS-PAGE and GeLC-MS/MS was performed. d. The mass spectrometric data was analyzed using a series of bioinformatics methods.
