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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Mol Immunol. 2011 Sep 25;49(1-2):297–303. doi: 10.1016/j.molimm.2011.08.024

Fig. 2. Effect of Oct-2 and OBF-1 on Ig expression and 3C interactions in MPC11 plasma cell line.

Fig. 2

A. RT-PCR detection of Oct-2 mRNA levels in GFP or Oct-2 shRNA-infected MPC11 cells. The levels of Oct-1 expression in the GFP- or Oct-2 shRNA treated cells are equivalent and both reduced as compared to the untreated MPC 11 cells. The levels of MPC11 H (γ2b) and L(kappa 19 and 21) chain expression were unchanged. Calreticulin (CalR) housekeeping gene was used for normalization of mRNA levels. EL-4 T cells were used for comparison. B. OBF-1 mRNA and protein levels were assessed in OBF-1 shRNA-treated MPC11 cells by RT-PCR (by three-fold dilutions) and Western blot, respectively. TFIID was used for protein normalization. C. 3C interactions were measured in EL-4 T cells, MPC11 cells, and GFP or Oct-2/OBF-1 shRNA infected MPC11 cells. No differences in interactions were observed with the KDs.