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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Mol Immunol. 2011 Sep 25;49(1-2):297–303. doi: 10.1016/j.molimm.2011.08.024

Fig. 3. Analysis of 3C interactions in pre-B cell lines.

Fig. 3

A. JH3-3’RR interactions were analyzed in thymocytes, 3-1 pre-B cells, and splenic B cells. Hole, the nearest non-Ig gene downstream of the 3’ RR, was used as a negative control. B. A schematic description of DJ/VDJ rearrangements on expressed (C57BL/6) and non-expressed (DBA) IgH alleles in 70Z/3 pre-B cell line. The J1 and hs4 primers were paired to detect 3C interaction only on the expressed allele. J3 and hs3a/hs1,2 were paired to detect 3C interactions only on the non-expressed allele. 3C interactions were measured in T cells from DBA mice (negative control), 70Z/3 pre-B cells, and DBA splenic B cells (positive control). Specific interactions were observed on the expressed allele of 70Z/3 pre-B cells.