Table 1.
Number of embryos | Stage at start of experiment | Duration | Stage at end of experiment | Time interval between imaging (min) | Z levels imaged | Estimated total energy load per embryo | Number developing normally |
10 | Morula | 22 hours | Blastocyst | 20 | 7 | 4 J | 0 |
12 | Morula | 22 hours | Blastocyst | 20 | 7 | 2.5 J | 0 |
8 | Morula | 22 hours | Blastocyst | 60 | 11 | 0.9 J | 1 |
8 | Morula | 24 hours | Blastocyst | not imaged | not imaged | ~ 0 J | 6 |
9 | Zygote | 10 min | Zygote | continuous | 10 | 16 J | 6 |
Embryos were subject to different imaging conditions and their development assessed. From the recipient with embryos subject to 0.9 J of energy, in addition to 1 normal embryo, 3 empty decidua and 2 resorbing embryos were found.