Table 2.
2DE-MS/MS identification of wheat flour proteins.
| Scaffold Assignments1 | Final Assignments2 | ||||||
|---|---|---|---|---|---|---|---|
| Protein type | Total Spots3 |
Total Sequences |
NCBI nr |
Contig databases4 |
Butte 86 Contigs or ESTs5 |
Final Sequences |
Butte 86 Contigs or ESTs5 |
| HMW-GS | 43 | 6 | 4 | 2 | 0 | 5 | 0 |
| LMW-GS | 35 | 24 | 9 | 4 | 11 | 22 | 10 |
| Gamma-gliadins | 34 | 13 | 2 | 1 | 10 | 13 | 10 |
| Omega-gliadins | 13 | 8 | 2 | 2 | 4 | 7 | 4 |
| Alpha-gliadins | 33 | 34 | 8 | 16 | 10 | 23 | 16 |
| Farinins | 11 | 3 | 0 | 0 | 3 | 3 | 3 |
| Purinins | 6 | 3 | 0 | 0 | 3 | 3 | 3 |
| Triticins | 9 | 3 | 0 | 3 | 0 | 3 | 0 |
| Globulins | 11 | 8 | 1 | 4 | 3 | 8 | 3 |
| GSPs/Puroindolines | 4 | 3 | 3 | 0 | 0 | 3 | 0 |
| Alpha-amylase inhibitors | 21 | 16 | 9 | 7 | 0 | 16 | 0 |
| Serpins | 15 | 7 | 2 | 5 | 0 | 9 | 6 |
| Beta-amylases | 12 | 4 | 0 | 1 | 3 | 4 | 3 |
| Other enzymes6 | 42 | 30 | 12 | 18 | 0 | 33 | 0 |
| Other proteins | 15 | 6 | 3 | 2 | 1 | 5 | 1 |
| Total | 168 | 55 | 65 | 48 | 157 | 59 | |
Numbers of spots and protein sequences and their database origins for each major protein category.
1Scaffold assignments for each spot are in Additional files 4, 5, 6, 7, 8, 9.
2Final assignments for each spot are in Additional file 1.
3Includes all 2-DE spots in which two or more peptides corresponded to sequences for the indicated protein type.
4Includes TaGI Releases 10.0 and 11.0, US Wheat Genome Project Assembly, HarvEST 1.14 and NCBI Unigene Build #55.
5Sequences derived from Butte 86 contigs are in Additional files 2, 3. Butte 86 sequences for some protein types were not analyzed in this study.
6Includes sequences for nine enzymes that were minor components of other spots as detailed in Additional file 1.