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. 2011 Feb 11;9:10. doi: 10.1186/1477-5956-9-10

Table 5.

Identification and quantification of gamma-gliadins by 2DE-MS/MS.

Spot # Predominant protein1 Total
proteins
in spot2 		Spot
Volume3 		Std
Dev4 		MS/MS
Coverage5,8 		Predicted
MW6,8 		pI7,8
325 Gamma-gliadin Bu-1 or -8 3 0.89 0.044 inc inc inc
326 Gamma-gliadin Bu-1 2 0.13 0.014 26 35521 7.7
160 Gamma-gliadin Bu-2 1 2.17 0.002 39 35188 8.2
166 Gamma-gliadin Bu-49 3 0.41 0.024 42 32606 8.2
169 Gamma-gliadin Bu-49 3 1.97 0.107 56 32606 8.2
337 Gamma-gliadin Bu-49 5 0.28 0.104 63 32606 8.2
134 Gamma-gliadin Bu-5 2 0.12 0.022 37 38943 7.8
320 Gamma-gliadin Bu-5 1 2.27 0.085 50 38943 7.8
323 Gamma-gliadin Bu-5 2 0.69 0.045 56 38943 7.8
324 Gamma-gliadin Bu-5 1 0.22 0.012 55 38943 7.8
335 Gamma-gliadin Bu-6 2 0.24 0.054 47 30616 8.5
346 Gamma-gliadin Bu-6 1 1.67 0.168 49 30616 8.5
347 Gamma-gliadin Bu-6 2 <0.01 0.000 32 30616 8.5
339 Gamma-gliadin Bu-7 2 0.36 0.039 52 31010 8.2
163 Gamma-gliadin Bu-11/[GenBank:AAD30556] 1 0.38 0.037 14 32018 6.5
527 Gamma-gliadin Bu-11/[GenBank:AAD30556] 3 0.37 0.018 32 32018 6.5

1Protein that matched the greatest number of peptides detected in spot by MS/MS. For partial Butte 86 contigs, the accession number for the most similar complete sequence is also indicated.

2-7Footnotes as in Table 3.

8inc indicates that sequence was incomplete.

9Contains nine Cys residues.