Table 6.
Identification and quantification of omega-gliadins by 2DE-MS/MS.
| Spot # | Predominant protein1 | Total proteins in spot2 |
Spot Volume3 |
Std Dev4 |
MS/MS Coverage5,8 |
Predicted MW6,8 |
pI7,8 |
|---|---|---|---|---|---|---|---|
| Omega-gliadins ω-5, 1B type (Gli-B3h) | |||||||
| 60 | Omega-gliadin ω-5 type TC11_288652 | 1 | 1.39 | 0.126 | 14 | 50904 | 6.0 |
| 63 | Omega-gliadin ω-5 type TC11_288652 | 1 | 0.19 | 0.025 | 13 | 50904 | 6.0 |
| 69 | Omega-gliadin ω-5 type9 | 1 | 0.18 | 0.006 | inc | inc | inc |
| 71 | Omega-gliadin ω-5 type TC11_288652 | 1 | 1.27 | 0.171 | 26 | 50904 | 6.0 |
| 73 | Omega-gliadin ω-5 type9 | 1 | 0.28 | 0.028 | inc | inc | inc |
| 74 | Omega-gliadin ω-5 type TC11-288652 | 1 | 1.77 | 0.235 | 30 | 50904 | 6.0 |
| Total | 5.08 | ||||||
| Omega-gliadins ω-1/ω-2, 1D type (Gli-D3a) | |||||||
| 476 | Omega-gliadin Gli-D3 type10 | 1 | 2.07 | 0.136 | inc | inc | inc |
| 477 | Omega-gliadin Bu-D1/[GenBank:AAT74547] | 3 | 0.99 | 0.087 | 23 | 43525 | 6.0 |
| Total | 3.06 | ||||||
| Omega-gliadins 1D Cys type | |||||||
| 107 | Omega-gliadin TC262770 | 1 | 0.12 | 0.015 | 8 | 41811 | 5.0 |
| 113 | Omega gliadin TC262770 | 2 | 0.50 | 0.012 | 46 | 41811 | 5.0 |
| 115 | Omega gliadin TC262770 | 2 | 0.47 | 0.065 | 34 | 41811 | 5.0 |
| 116 | Omega gliadin TC262770 | 1 | 0.16 | 0.024 | 8 | 41811 | 5.0 |
| Total | 1.25 | ||||||
| Omega-gliadins ω-1/ω-2, 1A type (Gli-A3f) | |||||||
| 130 | Omega-secalin-like [GenBank:ACN96903] | 1 | 0.08 | 0.055 | 27 | 39492 | 7.0 |
| 135 | Omega-gliadin Bu-D5 | 1 | 0.56 | 0.055 | inc | inc | inc |
| 391 | Omega-gliadin Bu-D5 | 2 | 0.43 | 0.049 | inc | inc | inc |
| Total | 1.07 |
Loci are indicated within parentheses.
1-7Footnotes as in Table 4.
8inc indicates sequence was incomplete.
9 No peptides were obtained; identified by other means.
10 Beta-amylase was the predominant protein detected in spot 476 but other data indicate that this spot was predominantly omega-gliadin.