Table 4.

Deduced amino acid differences between each high virulence WEEV strain (TBT235 and McMillan) and both low virulence WEEV strains 71V-1658 and CO92-1356

Protein	Amino acid positiona	Low virulence phenotype		High virulence phenotypeb
		71V-1658	CO92	TBT235	McMillan
nsP1	59	Q	Q	Q	E
	467	K	K	K	R
	513	P	P	S	P
nsP2	39	A	A	A	E
	433	T	T	I	T
	630	K	K	K	N
nsP3c	56	R	R	K	R
	289	L	L	L	P
	332	P	P	P	S
	334	A	A	V	T
	365	L	L	F	F
	418	V	V	V	D
	447	K	K	K	R
	469	Y	Y	H	H
nsP4	24	E	E	E	D
	27	L	L	L	F
	28	D	D	D	E
	147	A	A	A	S
	159	L	L	L	I
	537	P	P	S	P
	602	S	S	S	N
capsid	57	S	S	S	A
	74	K	K	K	E
	250	R	R	W	K
	252	T	T	P	T
E2	23	T	T	T	A
	69	F	F	F	Y
	146	H	H	H	Q
	157	H	H	H	R
	181	E	E	E	K
	214	R	R	R	Q
	224	K	K	K	R
	231	S	S	S	L
6K	30	V	V	V	I
E1	50	K	K	Q	K
	349	V	V	V	T
	374	S	S	S	T

^aAmino acid position number corresponds to WEEV strain 71V-1658.

^bBoldface type specifies amino acid residues unique to the high virulence WEEV strains when compared to both low virulence WEEV strains.

^cThere was an amino acid insertion of D at nsP3-167 in the McMillan strain and a deletion of V at nsP3-352 in the TBT235 strain.