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. 2011 May 20;3(1):e5. doi: 10.4081/hr.2011.e5

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©Copyright W. Hankey et al., 2011

This work is licensed under a Creative Commons Attribution 3.0 License (by-nc 3.0).

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Oligonucleotide pulldown analysis of C/EBPα and sumoylated-C/EBPα during ATRA-induction of MPRO cells. Nuclear extracts prepared from uninduced (0) and ATRA-induced (48h) MPRO cells were incubated with biotinylated C/EBP site (LF/C/EBP) oligomers from the LF promoter and OPD conducted as above. The blot was probed sequentially with anti-C/EBPα (lanes 3 and 4, top panel) and anti-SUMO-1 (lanes 3 and 4, bottom panel) antibodies. Input samples are represented in lanes 1 and 2 of the top (C/EBPα) and bottom (SUMO-1) panels.