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. 2011 May 20;3(1):e5. doi: 10.4081/hr.2011.e5

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©Copyright W. Hankey et al., 2011

This work is licensed under a Creative Commons Attribution 3.0 License (by-nc 3.0).

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Binding of Sumoylated-C/EBPA to the LF C/EBPα oligomers in ATRA-induced NB4 leukemic cells: A. Chromatin immunoprecipitation was performed from uninduced (0) and ATRA-induced (48h) NB4 cells using antibodies specific for C/EBPα . The precipitated chromatin was analyzed using primers specific for the human LF (LF) promoter (bottom panel) and the HNP promoter (top panel). This experiment was repeated three times. B. Oligonucleotide pulldown assay of C/EBPα and sumoylated-C/EBPα during ATRA-induction of NB4 cells. Nuclear extracts prepared from uninduced (0) and 48h ATRA-induced NB4 cells were incubated with biotinylated C/EBP site (LF/C/EBP) oligomers from the LF promoter and oligonucleotide pulldown assay conducted as above. The blot was probed sequentially with anti-C/EBPα (top panel) and anti-SUMO-1 (bottom panel) antibodies. + represents a positive control (uninduced MPRO extracts).