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(a) Effect of [MgATP] on the pyruvate carboxylation reaction in the absence of acetyl CoA (see Materials and Methods for reaction conditions). The solid line represents a non-linear least squares regression fit of the data to equation (iii). (b) Effect of [MgATP] on the bicarbonate-dependent ATP cleavage reaction in the absence of acetyl CoA (see Materials and Methods for reaction conditions). The line represents a non-linear least squares regression fit of the data to equation (iv).
