Figure 7.
Fluorescence emission spectra of 5 μM MgTNP-ATP in 0.1M Tris-Cl, pH7.8, 20 mM NaHCO3 (i) alone and in the presence of: (ii) 10 μM pyruvate carboxylase; (iii) 10 μM pyruvate carboxylase + 0.25 mM acetyl CoA; (iv) 10 μM pyruvate carboxylase and 0.25 mM acetyl CoA and 2.5 mM MgATP. Excitation wavelength = 408 nm, temperature = 30°C.