Figure 3.

Relationship between the induction of γH2AX or activation of ATM and the extent of EdU incorporation of cells exposed to H₂O_2. Panel A: The cells were exposed to EdU for 60 min and then to H₂O₂ for the next 60 min (as in Fig. 1C). The analysis gate was set in mid-S to exclude the enS and exS cells. Such analysis enabled one to correlate the extent of EdU incorporation of the cells that were exposed to EdU for the full 120 min at the time when they were replicating DNA compared to the expression of γH2AX (D) or ATM-S1981^P(E). [Color figure can be viewed in the online issue which is available at wileyonlinelibrary.com]