Figure 3. Effects of tissue-specific overexpression of dPGC-1 on fly longevity.

UAS-dPGC-1 was crossed to Gene-Switch (GS) driver lines (A) the ubiquitous Tubulin (tub)-GS driver, (B) the abdominal fat and digestive tract driver S1₁06, (C) the digestive tract driver TIGS-2 and (D) the pan-neuronal driver ELAV-GS, and lifespan curves are shown as induced (5 μg mL⁻¹ RU486 during development and 25 μg mL⁻¹ RU486 from the onset of adulthood (black circles) added on top of the food or uninduced (–RU486, open circles). (A) Lifespan curves of UAS-dPGC-1/tub-GS females. A moderate decrease in survival was observed in response to RU486 (P = 0.0029). (B) Lifespan curves of UAS-dPGC-1/S1₁06 females. An 11% increase in mean survival was observed in response to RU486 (P < 0.0001). (C) Lifespan curves of UAS-dPGC-1/TIGS-2 females. A 33% increase in mean survival was observed in response to RU486 (P < 0.0001). (D) Lifespan curves of UAS-dPGC-1/ELAV-GS females. No impact on survival was observed in response to RU486 (P = 0.8). The significance of the difference between survival curves was analyzed using log-rank statistical test (n>195 flies).

Survival data for male flies can be found in Figure S3. Survival data for independent insertions of dPGC-1 can be found in Figure S4. Survival data for control flies exposed to RU486 can be found in Figures S5 and S6.