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Slices of single axis tomograms of yeast cell sections prepared by freeze-substitution (a) and cryo-sectioning of vitrified samples (b). The black dots are mainly ribosomes. R, ribosomes; N, nucleus; V, vacuole; MT, microtubules (ring shaped cross sections); M, mitochondrion; ER, endoplasmic reticulum. Panel (b) was produced by Dan Clare.
