Figure 6.

Inversion in line A165 induced by SLCT. (A) SLCT model for formation of A165 is depicted in two steps. The parent chromosome is cut by Ac transposase at the 5′-end of Ds-y1 and at the 3′-end of Ds-y2, as shown by the short black arrows in the top diagram. Subsequently, the sequences flanking the 5′ and 3′ Ds termini are ligated, which results in the formation of a footprint (X) and inversion of a segment including Ds-y1 and fragments containing e and f, as shown in the second diagram. The third diagram shows that the excised Ds termini are reinserted into a site between e and f, as shown by the red vertical arrow and dotted arrows in the second diagram. Consequently, inverted fragments containing f and e are joined to the 5′-end of Ds-y1 and to the 3′-end of Ds-y2, respectively. (B) Genomic structures of OsRLG5 locus in parent and A165 lines. Short horizontal arrows (numbered) indicate the orientations and approximate positions of the primers at the OsRLG5 locus. Short vertical line between two Ds elements indicates the e-f target site. The orientation of ITS in the parent is indicated by the arrow. Gel photo shows the products of five PCR reactions using genomic A165 DNA template and the primer pairs indicated above each lane.