Figure 7.

Transposition-induced deletions in the OsRLG5 locus. (A) Southern blot analysis of lines E106 and 57. Genomic DNA was cut with SacI and hybridized with genomic probe 5B (Figure 3). Chromosomal rearrangement in line E106 was induced by Ds-y1 reinsertion in the proximal region. Ds-y2 was not excised from these two lines. Shorter DNA fragments are detected in these two lines compared with the parental line. (B) Southern blot analysis of deletions in the proximal region. Genomic DNA from the indicated lines was cut with EcoRI and hybridized with probe 5B. Sizes of hybridizing bands are shown at the left of the Southern blot. (C) Southern blot analysis of lines 220 and 285 which contain distal deletions. Genomic DNA was cut with EcoRI and hybridized with probe 5B. Deletion lines produce 4.2-kb fragments which are <5.3-kb band in parental lane. (D) Inferred structures of parental chromosome and distal deletions. Short vertical line between c and d indicates insertion target site. S indicates SacI digestion sites, whose positions are indicated. Ds-y1 was retained in line B89, but excised in line B48. The breakpoint of line B60 was not obtained. (E) SLCT-induced deletions distal to the original Ds position were examined by Southern blot. SacI cut genomic DNA was hybridized with probe 5A (Figure 3). Deletion chromosomes produced shorter bands than the parental line. W, wild-type; P, parental line.